Human EGFR (erbb1) Recombinant
Category: Surface & Soluble Receptors$160.00 – $1,950.00
Description
Accession
P00533
Source
DNA sequence encoding extracellular domain of Human EGFR (erbb1) to a C-terminal polyHis tag was expressed in Insect cells.
Molecular weight
Recombinant Human EGFR is a monomer consisting of 626 amino acid residue , and migrates as an approximately 75 kDa protein under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE
Biological Activity
The activity was determined by the ability to bind human EGF in functional ELISA.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human EGFR was lyophilized from a 0.2 μm filtered 20mM Tris,50mM NaCl, pH 7.4.
Reconstitution
A quick spin of the vial followed by reconstitution in sterile PBS pH 7.4 to a concentration not less than 0.1 mg/mL. Solubilize for 1hr at room temp with gentle mixing.
Storage
Recombinant EGFR can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months.It is recommended to add 0.1% HSA or BSA for long term storage. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
O14964
Interactor
Interactor
Interactor
Interactor
P22681
Interactor
P51149
Interactor
P62988
Interactor
P62991
Interactor
Q13596
Interactor
Q9UKV8
Interactor
Q9UPY3
Interactor
Interactor
Interactor
Interactor
Interactor
P29353
Interactor
Interactor
P62993
Interactor
Q03135
Interactor
Q13191
Interactor
Q6NZI2
Interactor
Q9UJM3
Molecular function
Molecular function
Molecular function
