| accession | P09228 |
| Source | Optimized DNA sequence encoding Human Cystatin SA-CST2(TRP21 - ALA141) mature chain including a C-terminal His tag was expressed in HEK293 cells. |
| Molecular weight | Recombinant Human Cystatin SA (CST2) is a protein consisting of 127 amino acid residue subunits,due to glycosylation migrates as an approximately as 17kDa band protein on reduced SDS-PAGE. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The activity was tested by the ability to inhibit Cathepsin L cleavage of a fluorogenic substrate. The ED50 was measured to be 10nM |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant Cystatin SA is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | Recombinant Human Cystatin SA (CST2) can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles. |
| Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Molecular function | Protease-inhibitor |
Human Cystatin SA Recombinant
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