Human Cystatin SA Recombinant

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Human Cystatin SA Recombinant


accession P09228

Source Optimized DNA sequence encoding Human Cystatin SA-CST2(TRP21 - ALA141) mature chain including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight Recombinant Human Cystatin SA (CST2) is a protein consisting of 127 amino acid residue subunits,due to glycosylation migrates as an approximately as 17kDa band protein on reduced SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The activity was tested by the ability to inhibit Cathepsin L cleavage of a fluorogenic substrate.

The ED50 was measured to be 10nM
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Cystatin SA is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage Recombinant Human Cystatin SA (CST2) can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Molecular function Protease-inhibitor