Human CXCL14 Recombinant
$60.00 – $2,500.00
| accession | O95715 |
| Source | Optimized DNA sequence encoding Human CXCL14 mature chain was expressed in ECOLI. Recombinant Human C-X-C motif chemokine 14 encodes amino acid residues 35-111. |
| Molecular weight | Recombinant CXCL14 migrates as an approximately 8 kDa protein under reducing conditions in SDS-PAGE. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | Determined by its ability to chemoattract activated monocytes using a concentration range of 1.0-10.0 ng/ml. |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | CXCL14 was Lyophilized from PBS, pH 7.2. |
| Sequence | SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIITTKS VSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this product can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Biological Process | Chemotaxis |
| Molecular function | Cytokine |
Methods
In vitro proliferation assays
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