Human CD32a Recombinant ( FcgRIIa), Biotinylated

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Human CD32a Recombinant ( FcgRIIa), Biotinylated


accession P12318

Source Optimized DNA sequence encoding Human extracellular domain of human CD32aincluding a C-terminal 6His tag was expressed in HEK293 cells. CD32a was further labled by Biotin for detection by avidin conjugated secondary probes.
Molecular weight Recombinant CD32a is a monomer protein consisting of 192 amino acid residue subunits, due to glycosylation migrates as an approximately25 kDa protein on SDS-PAGE.
Applications ELISA,WB Biotinylated CD32a was designed to be used in single step detection in ELISA ,WB
Purity >95%, as determined by SDS-PAGE and HPLC
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant CD32a is supplied as a lyophilized 0.2 μm filtered PBS solution, pH7.2 .
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL.
Storage Recombinant CD32a, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Biological Process Immunity
Molecular function IgG-binding-protein
Molecular function Receptor