Human CD31 Recombinant (PECAM1)

//Human CD31 Recombinant (PECAM1)

Human CD31 Recombinant (PECAM1)


accession P16284

Source Optimized DNA sequence encoding Human extracellular domain of human CD31including a C-terminal 6His tag was expressed in HEK293 cells.
Molecular weight Recombinant CD31 is a monomer protein consisting of 585 amino acid residue subunits, due to glycosylation migrates as an approximately100 kDa protein on SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity Recombinant Human CD31 activity was determined by its ability to support the adhesion of Jurkat human leukemic T cells. When cells are added to recombinant human CD31 coated plates ( 3.3 μg/mL , 100 μL/well ) in the presence of PMA , approximately 50% - 65% will adhere specifically after 30 minutes at 37℃
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant CD31 is supplied as a 0.2 μm filtered PBS solution, pH7.2 .
Storage Recombinant CD31, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Biological Process Cell-adhesion
Biological Process Phagocytosis