//Human CD3εδ heterodimer Recombinant

Human CD3εδ heterodimer Recombinant

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$160.00$3,500.00

SKU: RKK07766 Tags: , , ,

Description

Accession
P07766
Source
Optimized DNA sequence encoding extracellular domain of Human CD3ε including a flag tag was co-expressed with aDNA sequence encoding the extracellular domain of CD3δ including a C-terminal His tag, were expressed in HEK293 cells.
Molecular weight
Recombinant CD3 ε- CD3δ is a heterodimer protein consisting of 270 amino acid residue subunits, due to glycosylation migrates as an approximately 20kDa and as 26kDa bands on reduced SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE and HPLC
Biological Activity
The activity was tested by the ability of immobilized recombinant human CD3 epsilon-CD3 delta heterodimer to bind Human OKT3 with a linear range of 5-20 ng/ml
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD3ε-CD3δ is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Storage
Recombinant CD3 heterodimer can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
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