Human Carboxypeptidase E Recombinant

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Human Carboxypeptidase E Recombinant


accession P16870

Source Optimized DNA sequence encoding peptidase domain of Human Carboxypeptidase E-CPE (GLU26-SER453 ) including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight Recombinant human NGAL is a protein consisting of 433 amino acid residue subunits,due to glycosylation migrates as an approximately 53kDa protein on reduced SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The activity was tested by the ability to cleave a peptide substrate, benzoylAROH, and was measured to be 10 nMole/min/ug .
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human Carboxypeptidase E (CPE) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Storage Recombinant CPE can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Molecular function Hydrolase