Human Carbonic anhydrase IX Recombinant

/, Cytokines/Human Carbonic anhydrase IX Recombinant

Human Carbonic anhydrase IX Recombinant


accession Q16790

Source Optimized DNA sequence encoding extracellular domain of human Carbonic anhydrase IX (GLN38 - ASP414) including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight Recombinant human CAIX is a protein consisting of 384 amino acid residue subunits,due to glycosylation migrates as an approximately as 54kDa band protein on reduced SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological activity The activity was tested by its esterase activity , the measured activity is 15 pmole/ug/min
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human Carbonic anhydrase IX is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage Recombinant Carbonic anhydrase IX can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor P01730 CD4_HUMAN
Molecular function Lyase