Human c-MET (HGFR) Recombinant
Category: Surface & Soluble Receptors$160.00 – $1,950.00
Description
Accession
P08581
Source
Optimized DNA sequence encoding extracellular domain of Hepatocyte growth factor receptor ,c-MET(GLU25 - THR932) including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight
Recombinant human c-MET is a heteredimer protein consisting of alpha and beta chains and contains 915 amino acid residue subunits,due to glycosylation migrates as an approximately 45kDa and 85kDa bands protein on reduced SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological activity
The activity was tested by the ability of immobilized recombinant human HGF to bind recombinant human cMET (HGFR), the calculated ED50 is 2ug/ml
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human c-MET is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
Recombinant HGF receptor can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
O88838
Interactor
Interactor
Interactor
Interactor
Molecular function
Molecular function