Human c-MET (HGFR) Recombinant

//Human c-MET (HGFR) Recombinant

Human c-MET (HGFR) Recombinant


accession P08581

Source Optimized DNA sequence encoding extracellular domain of Hepatocyte growth factor receptor ,c-MET(GLU25 - THR932) including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight Recombinant human c-MET is a heteredimer protein consisting of alpha and beta chains and contains 915 amino acid residue subunits,due to glycosylation migrates as an approximately 45kDa and 85kDa bands protein on reduced SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological activity The activity was tested by the ability of immobilized recombinant human HGF to bind recombinant human cMET (HGFR), the calculated ED50 is 2ug/ml
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human c-MET is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage Recombinant HGF receptor can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor O88838
Interactor P01730 CD4_HUMAN
Interactor P14210 HGF_HUMAN
Interactor Q04756 HGFA_HUMAN
Interactor Q08048 HGF_MOUSE
Molecular function Transferase
Molecular function Receptor