Human BMP5 Recombinant
$60.00 – $3,550.00
| accession | P22003 |
| Source | Optimized DNA sequence encoding Human Bone Morphogenetic protein-5 mature chain was expressed in Escherichia Coli. |
| Molecular weight | Native human Bone Morphogenetic protein-5 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 16 kDa. Recombinant BMP-5 is a disulfide-linked homodimeric protein consisting of 2x139 amino acid residue subunits, and migrates as an approximately 16 kDa protein under non-reducing and reducing conditions in SDS-PAGE. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Protein Sequence | MHLTVFLLKG IVGFLWSCWV LVGYAKGGLG DNHVHSSFIY RRLRNHERRE IQREILSILG LPHRPRPFSP GKQASSAPLF MLDLYNAMTN EENPEESEYS VRASLAEETR GARKGYPASP NGYPRRIQLS RTTPLTTQSP PLASLHDTNF LNDADMVMSF VNLVERDKDF SHQRRHYKEF RFDLTQIPHG EAVTAAEFRI YKDRSNNRFE NETIKISIYQ IIKEYTNRDA DLFLLDTRKA QALDVGWLVF DITVTSNHWV INPQNNLGLQ LCAETGDGRS INVKSAGLVG RQGPQSKQPF MVAFFKASEV LLRSVRAANK RKNQNRNKSS SHQDSSRMSS VGDYNTSEQK QACKKHELYV SFRDLGWQDW IIAPEGYAAF YCDGECSFPL NAHMNATNHA IVQTLVHLMF PDHVPKPCCA PTKLNAISVL YFDDSSNVIL KKYRNMVVRS CGCH |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant BMP-5 is presented as 0.2 μm filtered10mM sodium Citrate buffer, %10 glycerol solution pH 4.0. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Biological Process | Chondrogenesis |
| Biological Process | Differentiation |
| Biological Process | Osteogenesis |
| Molecular function | Cytokine |
| Molecular function | Developmental-protein |
| Molecular function | Growth-factor |
Methods
BMP and FGF are robust trophic factors for purified astrocytes.
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