| accession | P12644 |
| Source | Optimized DNA sequence encoding Human Bone Morphogenetic protein-4 mature chain was expressed in Escherichia Coli. |
| Molecular weight | Native human Bone Morphogenetic protein-4 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately kDa. Recombinant BMP-4 is a disulfide-linked monomeric protein consisting of amino acid residue subunits, and migrates as an approximately as a kDa protein under reducing conditions in SDS-PAGE. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED(50) was determined by its ability to induce alkaline phosphatase productionin mouse ATDC5 chondrogenic and was found to be in the range of.1-0.2 ug/ml. |
| Protein Sequence | MIPGNRMLMV VLLCQVLLGG ASHASLIPET GKKKVAEIQG HAGGRRSGQS HELLRDFEAT LLQMFGLRRR PQPSKSAVIP DYMRDLYRLQ SGEEEEEQIH STGLEYPERP ASRANTVRSF HHEEHLENIP GTSENSAFRF LFNLSSIPEN EVISSAELRL FREQVDQGPD WERGFHRINI YEVMKPPAEV VPGHLITRLL DTRLVHHNVT RWETFDVSPA VLRWTREKQP NYGLAIEVTH LHQTRTHQGQ HVRISRSLPQ GSGNWAQLRP LLVTFGHDGR GHALTRRRRA KRSPKHHSQR ARKKNKNCRR HSLYVDFSDV GWNDWIVAPP GYQAFYCHGD CPFPLADHLN STNHAIVQTL VNSVNSSIPK ACCVPTELSA ISMLYLDEYD KVVLKNYQEM VVEGCGCR |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant BMP-4was lyophilized from a.2 μm filtered20mM Na2CO3, pH. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Biological Process | Chondrogenesis |
| Biological Process | Differentiation |
| Biological Process | Osteogenesis |
| Molecular function | Cytokine |
| Molecular function | Developmental-protein |
| Molecular function | Growth-factor |
Methods
Isolation, culture and 48 h induction of neural stem cells
|
Growth Factors
|
Growth Factors
|
Growth Factors
|
Effects of Activin A on paraxial mesodermal differentiation of mouse iPS cells.
|
