Human Bone Morphogenetic protein-2 Recombinant
Categories: Recombinant Human CytokinesTGF-beta familyTGF-beta family$70.00 – $4,700.00
Description
Accession
P12643
Source
Optimized DNA sequence encoding Human Bone Morphogenetic Protein-2 mature chain was expressed in Escherichia Coli.
Molecular weight
Native human Bone Morphogenetic Protein-2 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 13 kDa. Recombinant BMP-2 is a disulfide-linked homodimeric protein consisting of two 115 amino acid residue subunits.BMP2 migrates as an approximately 25 kDa protein under non-reducing conditions and as a 13 kDa protein under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determinedby its ability to induce alkaline phosphatase production by C2C12 myogenic cells cells was found to be less than ng/ml.
Protein Sequence
MVAGTRCLLA LLLPQVLLGG AAGLVPELGR RKFAAASSGR PSSQPSDEVL SEFELRLLSM FGLKQRPTPS RDAVVPPYML DLYRRHSGQP GSPAPDHRLE RAASRANTVR SFHHEESLEE LPETSGKTTR RFFFNLSSIP TEEFITSAEL QVFREQMQDA LGNNSSFHHR INIYEIIKPA TANSKFPVTR LLDTRLVNQN ASRWESFDVT PAVMRWTAQG HANHGFVVEV AHLEEKQGVS KRHVRISRSL HQDEHSWSQI RPLLVTFGHD GKGHPLHKRE KRQAKHKQRK RLKSSCKRHP LYVDFSDVGW NDWIVAPPGY HAFYCHGECP FPLADHLNST NHAIVQTLVN SVNSKIPKAC CVPTELSAIS MLYLDENEKV VLKNYQDMVV EGCGCR
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Bone Morphogenetic Protein-2 was lyophilized from a 0.2 μm filtered solution inmM AcOH pH.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
O88273
Interactor
Interactor
Interactor
Interactor
Interactor
Interactor
P97798
Biological Process
Biological Process
Biological Process
Molecular function
Molecular function
Molecular function
