Human beta Defensin-4 Recombinant
$70.00 – $2,700.00
accession | Q8WTQ1 |
Source | Optimized DNA sequence encoding Humanbeta Defensin-4 mature chain was expressed in Escherichia Coli. |
Molecular weight | Native humanBD-4 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately kDa. Recombinant beta Defensin-4 is a monomer protein consisting of51 amino acid residue subunits, and migrates as an approximately kDa protein under non-reducing and reducing conditions in SDS-PAGE. |
Purity | >98%, as determined by SDS-PAGE and HPLC |
Biological Activity | The ED(50) was determined by its ability to chemoattract immature human dendritic cells using a concentration range of-50 ng/ml. |
Protein Sequence | MQRLVLLLAI SLLLYQDLPV RSEFELDRIC GYGTARCRKK CRSQEYRIGR CPNTYACCLR KWDESLLNRT KP |
Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
Presentation | RecombinantHuman betaDefensin-4 was lyophilized from.2 μm filtered100mM NaCl,mM PB, pH.4. |
Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
Molecular function | Antimicrobial |