Human BCA-1 (CXCL13) Recombinant

///Human BCA-1 (CXCL13) Recombinant

Human BCA-1 (CXCL13) Recombinant

$70.00$2,700.00

SKU: RKQ53X90 Category: Tags: , , , , ,

accession Q53X90


Source Optimized DNA sequence encoding Human BCA-1 (CXCL13) mature chain was expressed in Escherichia Coli.
Molecular weight Human CXCL13, generated by the proteolytic removal of the signal peptide and propeptide and has a calculated molecular mass of approximately10 kDa. RecombinantCXCL13 is a disulfide-linked monomeric protein consisting of amino acid residue subunits and migrates as an approximately10 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity >98%, as determined by SDS-PAGE and HPLC
Biological Activity Determined by its ability to chemoattract human CXCR5 transfected mouse BaF3 B cells ,the ED50 is less thanng/ml.

Protein Sequence MKFISTSLLL MLLVSSLSPV QGVLEVYYTS LRCRCVQESS VFIPRRFIDR IQILPRGNGC PRKEIIVWKK NKSIVCVDPQ AEWIQRMMEV LRKRSSSTLP VPVFKRKIP
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant BCA-1 was lyophilized from a.2 μm filteredmM PB,100mM NaCl solution pH.5.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.



Methods

Level of serum CXCL13 in lung cancer (LuCa) patients.

CXCL13
  • Serum CXCL13 levels in normal healthy donors (n=9) and patients diagnosed with squamous cell carcinoma (n=17) or adenocarcinoma (n=14) were analyzed by a quantitative enzyme-linked immunosorbent assay technique, which was capable of detecting >5 pg/ml.