Human basic FGF Recombinant, Biotinylated
$340.00 – $1,950.00
| accession | P09038 |
| Source | Optimized DNA sequence encoding Human basic Fibroblast Growth Factor mature chain was expressed in Escherichia Coli. |
| Molecular weight | Native human FGF basic is generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated mass of 17 kDa. Recombinant bFGF is a monomer protein consisting of 155 amino acid residue subunits, and migrates as an approximately 17 kDa protein under non-reducing and reducing conditions in SDS-PAGE. |
| Purity | >96%, as determined by SDS-PAGE and HPLC |
| Applications | ELISA,WB Biotinylated FGF basic was designed to be used in single step detection in ELISA ,WB |
| Protein Sequence | MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Human Basic FGF was lyophilized from a 0.2 μm filtered solution inM NaCl,20mM PB pH7.0 . |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | O35082 |
| Interactor | O43353 |
| Interactor | P21802 FGFR2_HUMAN |
| Interactor | P22607 FGFR3_HUMAN |
| Interactor | Q802A9 |
| Interactor | P11362 FGFR1_HUMAN |
| Interactor | P22455 FGFR4_HUMAN |
| Biological Process | Angiogenesis |
| Biological Process | Differentiation |
| Molecular function | Developmental-protein |
| Molecular function | Growth-factor |
| Molecular function | Heparin-binding |