Human basic FGF Recombinant, Biotinylated
$340.00 – $1,950.00
accession | P09038 |
Source | Optimized DNA sequence encoding Human basic Fibroblast Growth Factor mature chain was expressed in Escherichia Coli. |
Molecular weight | Native human FGF basic is generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated mass of kDa. Recombinant bFGF is a monomer protein consisting of 155 amino acid residue subunits, and migrates as an approximately 17 kDa protein under non-reducing and reducing conditions in SDS-PAGE. |
Purity | >96%, as determined by SDS-PAGE and HPLC |
Applications | ELISA,WB Biotinylated FGF basic was designed to be used in single step detection in ELISA ,WB |
Protein Sequence | MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS |
Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
Presentation | Human Basic FGF was lyophilized from a.2 μm filtered solution inM NaCl,20mM PB pH7.0 . |
Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
Interactor | O35082 |
Interactor | O43353 |
Interactor | P21802 FGFR2_HUMAN |
Interactor | P22607 FGFR3_HUMAN |
Interactor | Q802A9 |
Interactor | P11362 FGFR1_HUMAN |
Interactor | P22455 FGFR4_HUMAN |
Biological Process | Angiogenesis |
Biological Process | Differentiation |
Molecular function | Developmental-protein |
Molecular function | Growth-factor |
Molecular function | Heparin-binding |