Human BAFF-R(CD268) Recombinant
Category: Surface & Soluble Receptors$70.00 – $1,200.00
Description
Accession
Q96RJ3
Source
DNA sequence encoding Human BAFF-R extracellular domain was expressed in HEK293 cells
Molecular weight
Recombinant human BAFF-R, is a monomeric protein consisting of amino acid residue subunits. The molecule has a calculated molecular mass of approximately 8kDa and migrates due to glycosylation as an approximately 20kDa protein under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE
Biological Activity
The ED(50) was determined by the ability to block BAFF induced survival of splenocyte cells, and was found to be in the range of.0-5.0 ug/ml.
Protein Sequence
MRRGPRSLRG RDAPAPTPCV PAECFDLLVR HCVACGLLRT PRPKPAGASS PAPRTALQPQ ESVGAGAGEA ALPLPGLL FG APALLGLALV LALVLVGLVS WRRRQRRLRG ASSAEAPDGD
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant BAFF-R was lyophilized from a.2 μm filtered solution in.5% glycine,.5% sucrose,.01% Tween80, mM Glutamic acid, pH.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function