Human BAFF (CD257) Recombinant

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Human BAFF (CD257) Recombinant

$70.00$4,700.00


accession Q9Y275


Source Optimized DNA sequence encoding Human BAFF extracellular domain was expressed in Escherichia Coli
Molecular weight Recombinant human Apolipoprotein E3, is a monomer protein consisting of amino acid residue subunits 134-285. The molecule has a calculated molecular mass of approximately 17kDa and migrates as an approximately 17kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Protein Sequence AVQGPEETVT QDCLQLIADS ETPTIQKGSY TFVPWLLSFK RGSALEEKEN KILVKETGYF FIYGQVLYTD KTYAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PRENAQISLD GDVTFFGALK LL
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant Human BAFF was lyophilized from a.2 μm filtered solution in PBS, pH.5.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor O14836 TR13B_HUMAN
Interactor O14836 TR13B_HUMAN
Biological Process Immunity
Molecular function Cytokine

Methods

RhoA is necessary for BAFF-mediated B cell survival but not proliferation.

recombinant human BAFF
  • Splenic B220+ B cells from control and RhoA mice were cultured for 72 hours on 96-well plates (2×105 cells/well) with or without 2 µg/mL anti-IgM F(ab')2 antibody or indicated concentrations of BAFF (left).

Identification of IL21, CD40L, αIgM, BAFF and LPS regulated genes in transformed human germinal centre B cells using microarrays.

human BAFF
  • BL2 cell were stimulated with αIgM F(ab)2 fragments (3 hrs) , IL21 (2 hrs) , CD40L (6 hrs) , LPS (6 hrs) and BAFF (9 hrs) .

(A) B cell activating factor (BAFF) mRNA expression in monocytes in patients with primary Sjögren's syndrome (pSS) (n=69) and healthy controls (HC) (n=44).

BAFF mRNA expression
  • BAFF mRNA expression was determined by real time quantitative-PCR.

(A) B cell activating factor (BAFF) mRNA expression in monocytes in patients with primary Sjögren's syndrome (pSS) (n=69) and healthy controls (HC) (n=44).

BAFF protein
  • BAFF mRNA expression was determined by real time quantitative-PCR.

Cell stimulation and total IgG production

  • PBMCs recovered from the gradient interface were washed twice in PBS and adjusted to 106 cells/mL in RPMI 1640 supplemented with 10% FCS and 50 μg/mL gentamicin (GIBCO).
  • Cells were cultured in the presence of 100 ng/mL rhIL-21 and/or 100 ng/mL rhBAFF for 12 days at 37°C with 5% CO2.
  • After 12 days, culture supernatants were collected and total IgG was measured using an in-house ELISA as described previously [