Human BAFF (CD257) Recombinant

/, CD markers/Human BAFF (CD257) Recombinant

Human BAFF (CD257) Recombinant

$70.00$4,700.00

SKU: RKQ9Y275 Categories: , Tags: , , , , , , , ,

accession Q9Y275


Source Optimized DNA sequence encoding Human BAFF extracellular domain was expressed in Escherichia Coli
Molecular weight Recombinant human Apolipoprotein E3, is a monomer protein consisting of amino acid residue subunits 134-285. The molecule has a calculated molecular mass of approximately 17kDa and migrates as an approximately 17kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Protein Sequence AVQGPEETVT QDCLQLIADS ETPTIQKGSY TFVPWLLSFK RGSALEEKEN KILVKETGYF FIYGQVLYTD KTYAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PRENAQISLD GDVTFFGALK LL
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant Human BAFF was lyophilized from a.2 μm filtered solution in PBS, pH.5.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biological Process Immunity
Molecular function Cytokine

Methods

RhoA is necessary for BAFF-mediated B cell survival but not proliferation.

recombinant human BAFF
  • Splenic B220+ B cells from control and RhoA mice were cultured for 72 hours on 96-well plates (2×105 cells/well) with or without 2 µg/mL anti-IgM F(ab')2 antibody or indicated concentrations of BAFF (left).

Identification of IL21, CD40L, αIgM, BAFF and LPS regulated genes in transformed human germinal centre B cells using microarrays.

human BAFF
  • BL2 cell were stimulated with αIgM F(ab)2 fragments (3 hrs) , IL21 (2 hrs) , CD40L (6 hrs) , LPS (6 hrs) and BAFF (9 hrs) .

(A) B cell activating factor (BAFF) mRNA expression in monocytes in patients with primary Sjögren's syndrome (pSS) (n=69) and healthy controls (HC) (n=44).

BAFF mRNA expression
  • BAFF mRNA expression was determined by real time quantitative-PCR.

(A) B cell activating factor (BAFF) mRNA expression in monocytes in patients with primary Sjögren's syndrome (pSS) (n=69) and healthy controls (HC) (n=44).

BAFF protein
  • BAFF mRNA expression was determined by real time quantitative-PCR.

Cell stimulation and total IgG production

  • PBMCs recovered from the gradient interface were washed twice in PBS and adjusted to 106 cells/mL in RPMI 1640 supplemented with 10% FCS and 50 μg/mL gentamicin (GIBCO).
  • Cells were cultured in the presence of 100 ng/mL rhIL-21 and/or 100 ng/mL rhBAFF for 12 days at 37°C with 5% CO2.
  • After 12 days, culture supernatants were collected and total IgG was measured using an in-house ELISA as described previously [