Human Angiopoietin-2 Recombinant

/Human Angiopoietin-2 Recombinant

Human Angiopoietin-2 Recombinant

$160.00$2,150.00

SKU: RKO15123 Tags: , , ,

accession O15123


Source Optimized DNA sequence encoding extracellular domain of Human Angiopoietin-2 including a C-terminal His tagwas expressed in HEK293 cells.
Molecular weight RecombinantAngpt2is a protein consisting of490 amino acid residue subunits, due to glycosylation migrates as an approximately 70kDa protein on SDS-PAGE.
Purity >97%, as determined by SDS-PAGE and HPLC
Biological Activity The activity was tested by the ability of immobilized recombinant Human Tie2 to bind biotinylated recombinant human Angiopoietin-2 in ELISA
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human Angiopoietin-2 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Storage Recombinant Angiopoietin-2 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor Q02763 TIE2_HUMAN
Biological Process Angiogenesis
Biological Process Differentiation
Molecular function Developmental-protein

Methods

Binding kinetics measurements by surface plasmon resonance

Ang2
  • The kinetics of Zybody binding to Ang2 was determined as follows.
  • Anti-His IgG was immobilized to the CM5 sensor chip using standard amine coupling procedure as recommended by the manufacturer.
  • Ang2 at 1 μg/ml concentration was captured onto the anti-His surface at low levels (~20–40U).
  • A range of concentrations (0.25 nM to 66 nM) of ADA-a2H were injected at 30 μl/min for 3 min with 10 min dissociation per cycle.
  • Binding kinetics was also determined with Ang2 prepared at Zyngenia using ADA-a2H captured on to a CM5 chip via goat anti-human kappa chain (Fab'2) .
  • Various concentrations of Ang2 (1.2 nM to 100 nM) were injected at 30 μl/min for 4 min with 10 min dissociation per cycle.
  • The surface was regenerated with 10 mM glycine, pH 1.5 after each cycle.
  • Binding data were analyzed using a 1:1 Langmuir model.