Human Angiopoietin-2 Recombinant, Biotinylated

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Human Angiopoietin-2 Recombinant, Biotinylated


accession O15123

Source Optimized DNA sequence encoding extracellular domain of Human Angiopoietin-2 (TYR19-PHE496) including a C-terminal His tag was expressed in HEK293 cells. Recombinant Angiopoietin-2 was further labled by Biotin for detection by avidin conjugated secondary probes.
Molecular weight RecombinantAngpt2is a protein consisting of490 amino acid residue subunits, due to glycosylation migrates as an approximately 70kDa protein on SDS-PAGE.
Purity >97%, as determined by SDS-PAGE and HPLC
Biological Activity The activity was tested by the ability of immobilized recombinant Human Tie2 to bind biotinylated recombinant human Angiopoietin-2 in ELISA
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human Angiopoietin-2 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Storage Recombinant Angiopoietin-2 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor Q02763 TIE2_HUMAN
Biological Process Angiogenesis
Biological Process Differentiation
Molecular function Developmental-protein