Human alpha 1 antichymotrypsin Recombinant

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Human alpha 1 antichymotrypsin Recombinant


accession P01011

Source Optimized DNA sequence encodingHuman alpha 1 antichymotrypsin -AACT (HIS23 - ALA423) mature chain including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight Recombinant Humanalpha 1 antichymotrypsin(AACT) is a protein consisting of 410 amino acid residue subunits,due to glycosylation migrates as an approximately as 46kDa band protein on reduced SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The activity was measured by the ability of inhibit chymotrypsin cleavage of a fluorogenic peptide substrate, and the calulated ED50 was found to be < 6nM
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant human alpha 1 antichymotrypsin(AACT) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage Recombinant Human alpha 1 antichymotrypsin (AACT) can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Biological Process Acute-phase
Molecular function Protease-inhibitor