Human Adiponectin Recombinant

Human Adiponectin Recombinant

$160.00$3,500.00


accession Q15848


Source Optimized DNA sequence encoding extracellular domain of Human Adiponectin (19-244) including a C-terminal His tag was expressed in CHO cells.
Molecular weight Recombinant Adiponectin is a protein consisting of 226 amino acid residue subunits, due to glycosylation migrates as an approximately 35kDa protein on SDS-PAGE.
Purity >97%, as determined by SDS-PAGE and HPLC
Biological Activity The activity was tested by Determined by a cytotoxic assay using M1 cells. The ED50 for this effect is 3.0-6.0 μg/ml.
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human Adiponectin is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Storage Recombinant Adiponectin can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Molecular function Hormone

Methods

Globular adiponectin induces apoptosis in HepG2 cells.

gAcrp
  • Cells were treated with gAcrp (1 μg/ml) for the indicated time points.