Human acidic Fibroblast Growth Factor Recombinant
$70.00 – $1,350.00
| accession | P05230 |
| Source | Optimized DNA sequence encoding Human acidic Fibroblast Growth Factor mature chain was expressed in Escherichia Coli. |
| Molecular weight | Native human aFGF, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated mass of 5 kDa. Recombinant FGF acidic is a monomer protein consisting of amino acids and migrates as an approximately 16 kDa protein under reducing and non-reducing conditions. |
| Purity | >98%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤0.1 ng/ml, corresponding to a specific activity of ≥ 1x10 7units/mg. |
| Protein Sequence | MAEGEITTFT ALTEKFNLPP GNYKKPKLLY CSNGGHFLRI LPDGTVDGTR DRSDQHIQLQ LSAESVGEVY IKSTETGQYL AMDTDGLLYG SQTPNEECLF LERLEENHYN TYISKKHAEK NWFVGLKKNG SCKRGPRTHY GQKAILFLPL PVSSD |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant acidic FGF was lyophilized from a 0.2 μm filtered solution in.5% glycine,.5% sucrose,.01% Tween80, mM Glutamic acid, pH.5. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P11362 FGFR1_HUMAN |
| Interactor | P22455 FGFR4_HUMAN |
| Interactor | P21802 FGFR2_HUMAN |
| Interactor | P22607 FGFR3_HUMAN |
| Interactor | Q8NI16 |
| Biological Process | Angiogenesis |
| Biological Process | Differentiation |
| Molecular function | Developmental-protein |
| Molecular function | Growth-factor |
| Molecular function | Heparin-binding |
Methods
R1MAb2 activates FGFR1 in the presence of Klotho.
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