Human ACE-2 Recombinant-Fc
Categories: 2019-nCoV/COVID-19 CoronavirusMERS CoronavirusRecombinant Human CytokinesSARS antigens$400.00 – $2,600.00
Description
Accession
Q9BYF1
Source
DNA sequence encoding the extracellular domain of Human ACE2 including a C-terminal human IgG1-Fc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human ACE2 is a protein consisting of amino acid residues [Gln 38-Ser740] ,due to glycosylation migrates as an approximately 116 kDa protein on SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant ACE2 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Sequence
DLFYQSSLAS WNYNTNITEE NVQNMNNAGD KWSAFLKEQS TLAQMYPLQE IQNLTVKLQL QALQQNGSSV LSEDKSKRLN TILNTMSTIY STGKVCNPDN PQECLLLEPG LNEIMANSLD YNERLWAWES WRSEVGKQLR PLYEEYVVLK NEMARANHYE DYGDYWRGDY EVNGVDGYDY SRGQLIEDVE HTFEEIKPLY EHLHAYVRAK LMNAYPSYIS PIGCLPAHLL GDMWGRFWTN LYSLTVPFGQ KPNIDVTDAM VDQAWDAQRI FKEAEKFFVS VGLPNMTQGF WENSMLTDPG NVQKAVCHPT AWDLGKGDFR ILMCTKVTMD DFLTAHHEMG HIQYDMAYAA QPFLLRNGAN EGFHEAVGEI MSLSAATPKH LKSIGLLSPD FQEDNETEIN FLLKQALTIV GTLPFTYMLE KWRWMVFKGE IPKDQWMKKW WEMKREIVGV VEPVPHDETY CDPASLFHVS NDYSFIRYYT RTLYQFQFQE ALCQAAKHEG PLHKCDISNS TEAGQKLFNM LRLGKSEPWT LALENVVGAK NMNVRPLLNY FEPLFTWLKD QNKNSFVGWS TDWSPYADQS IKVRISLKSA LGDKAYEWND NEMYLFRSSV AYAMRQYFLK VKNQMILFGE EDVRVANLKP RISFNFFVTA PKNVSDIIPR TEVEKAIRMS RSRINDAFRL NDNSLEFLGI QPTLGPPNQP
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Q6Q1S2
Biological Process
Molecular function
Molecular function
Methods
Recombinant Human ACE2 Fc SDS PAGE
- The recombinant ACE2-Fc protein migrates as 120kDa due to glycosylation
Activity assay Human ACE2 Fc-nCov S1
- Recombinant ACE2-Fc functional ELISA assay binding immobilized nCov Spike S1 protein
