//Human 2B4/CD244/SLAMF4 Protein Recombinant

Human 2B4/CD244/SLAMF4 Protein Recombinant

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$300.00$700.00

SKU: KTGEFSNF

Description

Accession
Q9BZW8
Source
DNA sequence encoding Human 2B4/CD244/SLAMF4 including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human 2B4/CD244/SLAMF4 is a protein consisting of amino acid residues [Cys22-Arg221] ,The protein has a predicted MW of 49 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human 2B4/CD244/SLAMF4 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Q14155
Biological Process
Molecular function

Methods

Human 2B4/CD244/SLAMF4 Protein Tris-Bis PAGE

  • Human 2B4/CD244/SLAMF4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Human 2B4/CD244/SLAMF4 Protein SEC-HPLC

  • The purity of Human 2B4/CD244/SLAMF4 is greater than 95% as determined by SEC-HPLC.

Human 2B4/CD244/SLAMF4 Protein ELISA Data

  • Immobilized Human CD48, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human 2B4, hFc Tag with the EC50 of 0.45μg/ml determined by ELISA.
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