| accession | HLAG |
| Source | DNA sequence encoding the Human (HLA-G AA25-305 [accession# P17693] and the B2M protein AA2-119 [accession# P61769], fused to the RIIPRHLQL peptide.This complex includes a C terminal polyHis AVI tag and was expressed in HEK cells, |
| Molecular weight | Recombinant HLA-G/RIIPRHLQL has a calculated mass of approximately 50kDa. Due to glycosylation migrates as an approximately 60 kDa protein under reducing conditions in SDS-PAGE. |
| Purity | purity was determined to be >95% by non reducing PAGE and HPLC (see below methods) |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant HLA-G/RIIPRHLQL was lyophilized from 0.2 µm filtered PBS solution pH7.4 |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. |
| Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
Methods
HLA-G RIIPRHLQL peptide complex PAGE
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HLA-G RIIPRHLQL peptide complex HPLC
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