HLA-A*03:01 KRAS G12V peptide Tetramer
Categories: HLA-A peptide complexesMHC peptide complexes$510.00 – $2,025.00
Description
Accession
KRAS
Source
DNA sequence encoding the Human (HLA-A*03:01) AA25-305 [accession# P04439] and the B2M protein AA2-119 [accession# P61769], fused to the mutant G12V KRAS epitope VVVGAVGVGK .This complex includes a C terminal polyHis AVI tag and was expressed in HEK cells, The teramer conformation was achieved through biotinylation of the monomer and then covalently binding the monomers via an avidin biotin reaction.
Molecular weight
Recombinant HLA-A/KRAS G12V has a calculated mass of approximately 250kDa. Due to glycosylation migrates as an approximately 260 kDa protein under reducing conditions in SDS-PAGE.
Purity
purity was determined to be >95% by nonreducing PAGE and HPLC (see below methods)
Activity
The activity was tested in an ELISA assay. Human KRAS G12V (HLA-A*03:01) Tetramer binds Anti KRSA G12V antibody with the EC50 of 0.6μg/ml .
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant HLA-A*03:01 KRAS G12V complex was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
KRAS G12V (HLA-A*03:01)Tetramer protein PAGE
- 1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.
KRAS G12V (HLA-A*03:01) Tetramer HPLC
- The purity of KRAS G12V (HLA-A*03:01)complex tetramer was greater than 95% as determined by SEC-HPLC.
KRAS G12V (HLA-A*03:01) Tetramer ELISA
- Dose response curve for Anti-KRAS G12V(HLA-A*03:01) Antibody
KRAS G12V (HLA-A*03:01) Tetramer SPR
- SPR image shows curve for KRAS G12V(HLA-A*03:01) and immobilized Antibody binding, with a calculated affinity constant of 42nM
