HLA-A*02:01 WT-1 peptide complex Tetramer

//HLA-A*02:01 WT-1 peptide complex Tetramer

HLA-A*02:01 WT-1 peptide complex Tetramer

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SKU: MHCWT1T Category:

accession WT1


Source DNA sequence encoding the Human (HLA-A*02:01) AA25-305 [accession# P04439] and the B2M protein AA2-119 [accession# P61769], fused to the Wilms tumor peptide RMFPNAPYL .This complex includes a C terminal polyHis AVI tag and was expressed in HEK cells, The teramer conformation was achieved through biotinylation of the monomer and then covalently binding the monomers via an avidin biotin reaction.
Molecular weight Recombinant HLA-A/WT1 has a calculated mass of approximately 250kDa. Due to glycosylation migrates as an approximately 260 kDa protein under reducing conditions in SDS-PAGE.
Purity purity was determined to be >95% by nonreducing PAGE and HPLC (see below methods)
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant HLA-A*02:01 WT1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.



Methods

HLA-A*02:01 Wilms Tumor peptide complex tetramer SPR

  • SPR image shows curve for HLA-A*02:01 Wilms Tumor WT1 tetramer and immobilized Antibody binding, with a calculated affinity constant of 0.3nM

HLA-A*02:01 Wilms Tumor WT1 Tetramer complex HPLC

  • The purity of Wilms Tumor WT1 (HLA-A*02:01)complex Tetramer was greater than 95% as determined by SEC-HPLC.

HLA-A*02:01 Wilms Tumor WT1 peptide complex Tetramer PAGE

  • 1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.

HLA-A*02:01 Wilms Tumor WT1 complex peptide complex tetramer ELISA

  • Dose response curve for Anti-HLA-A*02:01 Wilms Tumor WT1 Antibody