HLA-A*02:01 NY-ESO-1 peptide complex Tetramer
Categories: HLA-A peptide complexesMHC peptide complexes$510.00 – $2,025.00
Description
Accession
NYESO1
Source
DNA sequence encoding the Human (HLA-A*02:01) AA25-305 [accession# P04439] and the B2M protein AA2-119 [accession# P61769], fused to the mutant NY-ESO-1 epitope SLLMWITQC .This complex includes a C terminal polyHis AVI tag and was expressed in HEK cells, The teramer conformation was achieved through biotinylation of the monomer and then covalently binding the monomers via an avidin biotin reaction.
Molecular weight
Recombinant HLA-A/NY-ESO-1 has a calculated mass of approximately 250kDa. Due to glycosylation migrates as an approximately 260 kDa protein under reducing conditions in SDS-PAGE.
Purity
purity was determined to be >95% by nonreducing PAGE and HPLC (see below methods)
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant (HLA-A*02:01) NY-ESO1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
HLA-A*02:01 NY-ESO-1 peptide complex tetramer SPR
- SPR image shows curve for HLA-A*02:01 NY-ESO-1 tetramer and immobilized Antibody binding, with a calculated affinity constant of 15nM
HLA-A*02:01 NY-ESO-1 peptide complex tetramer ELISA
- Dose response curve for Anti-HLA-A*02:01 NY-ESO-1 Antibody
HLA-A*02:01 NY-ESO-1 peptide complex tetramer PAGE
- 1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.
NY-ESO-1 (HLA-A*02:01) HPLC
- The purity of NY-ESO-1 (HLA-A*02:01)complex was greater than 95% as determined by SEC-HPLC.
