Description
Source
Optimized DNA sequence encoding immunodominant sequences of the HIV1 Group M Inegrase fused to a C terminal GST tag was expressed in E.Coli.
Molecular weight
Recombinant HIV1 Integrase migrates as approx 42kDa protein on reduced SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant HIV-1 Integrase was lyophilized from a.2 μm filteredmm Tris solutionpH.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this product can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.