Cynomolgus ACE-2 Recombinant-His
Categories: 2019-nCoV/COVID-19 CoronavirusMERS CoronavirusRecombinant Human CytokinesSARS antigens$400.00 – $2,600.00
Description
Accession
B6DUF3
Source
DNA sequence encoding the extracellular domain of Rhesus ACE2 including a C-terminal His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Rhesus ACE2 is a protein consisting of amino acid residues [Gln 18-Val739] ,due to glycosylation migrates as an approximately 119 kDa protein on SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The activity was determined by its ability to bind in functional ELISA assay.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant ACE2 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Sequence
QSTIEEQAKT FLDKFNHEAE DLFYQSSLAS WNYNTNITEE NVQNMNNAGE KWSAFLKEQS TLAQMYPLQE IQNLTVKLQL QALQQNGSSV LSEDKSKRLN TILNTMSTIY STGKVCNPNN PQECLLLDPG LNEIMEKSLD YNERLWAWEG WRSEVGKQLR PLYEEYVVLK NEMARANHYK DYGDYWRGDY EVNGVDGYDY NRDQLIEDVE RTFEEIKPLY EHLHAYVRAK LMNAYPSYIS PTGCLPAHLL GDMWGRFWTN LYSLTVPFGQ KPNIDVTDAM VNQAWNAQRI FKEAEKFFVS VGLPNMTQGF WENSMLTDPG NVQKVVCHPT AWDLGKGDFR IIMCTKVTMD DFLTAHHEMG HIQYDMAYAA QPFLLRNGAN EGFHEAVGEI MSLSAATPKH LKSIGLLSPD FQEDNETEIN FLLKQALTIV GTLPFTYMLE KWRWMVFKDE IPKDQWMKKW WEMKREIVGV VEPVPHDETY CDPASLFHVS NDYSFIRYYT RTLYQFQFQE ALCQAAKHEG PLHKCDISNS TEAGQKLLNM LKLGKSEPWT LALENVVGAK NMNVRPLLNY FEPLFTWLKD QNKNSFVGWS TDWSPYADQS IKVRISLKSA LGDKAYEWND NEMYLFRSSV AYAMRTYFLE IKHQTILFGE EDVRVADLKP RISFNFYVTA PKNVSDIIPR TEVEEAIRIS RSRINDAFRL NDNSLEFLGI QTTLAPPYQS
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
