COV2 Spike protein S Recombinant B.1.1.529 (Omicron)-His

///COV2 Spike protein S Recombinant B.1.1.529 (Omicron)-His

COV2 Spike protein S Recombinant B.1.1.529 (Omicron)-His

$350.00$1,200.00


accession NCOVS1


Source DNA sequence encoding extracellular fragment[16-1220] of COV2 Spike S protein(S1+S2) [accession# QIC53204.1] variant (B.1.1.529/Omicron) (A67V, del69-70, T95I, del142-144, Y145D, del211, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F)including a C terminal polyHis tag was expressed in HEK cells.
Molecular weight Recombinant NCOV Spike protein B.1.1.529 encodes the NCOV spike glycoprotein S1+S2 sequences . Recombinant COV2 Spike migrates due to glycosylation as an approximately 130 kDa protein under reducing conditions in SDS-PAGE.
Activity The activity was tested by binding Human ACE2 in functional ELISA assay (see figure below).
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant COVID-19 Spike was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.



Methods

COV2 Spike B.1.1.529 omicron variant protein PAGE

  • 1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.

SARS COV2 Spike B.1.1.529/omicron ACE functional binding ELISA assay

  • COV2 S (coating at 0.1ug-well) binding with Human ACE2-Fc (cat#RKQ9BYF1F) .The linear range was found to be 100 ng/ml