COV2 Spike protein RBD Recombinant B.1.1.529 (Omicron)-His
$350.00 – $1,200.00
| accession | NCOVS1 |
| Source | DNA sequence encoding extracellular fragment[319-541] of COV2 Spike RBD domain [accession# QIC53204.1] variant (B.1.1.529/Omicron) ( G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H)including a C terminal polyHis tag was expressed in HEK cells. |
| Molecular weight | Recombinant COV2 RBD B.1.1.529 encodes the NCOV spike glycoprotein RBD domain sequence . This molecule has a calculated mass of approximately 76kDa. Recombinant COV2 RBD migrates due to glycosylation as an approximately 120 kDa protein under reducing conditions in SDS-PAGE. |
| Activity | The activity was tested by binding Human ACE2 in functional ELISA assay (see figure below). |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant COVID-19 Spike RBD was lyophilized from 0.2 µm filtered PBS solution pH7.4 |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. |
| Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
Methods
SARS COV-2 Spike RBD B.1.1.529 omicron HPLC
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RBD variant B.1.1.529 omicron protein PAGE
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SARS COV2 RBD B.1.1.529/omicron ACE functional binding ELISA assay
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