bovine Basic Fibroblast Growth Factor Recombinant

/, FGF family/bovine Basic Fibroblast Growth Factor Recombinant

bovine Basic Fibroblast Growth Factor Recombinant

$70.00$1,300.00

SKU: RKP03969 Categories: , Tags: , , ,

accession P03969


Source Optimized DNA sequence encodingBovine Basic Fibroblast Growth Factor mature chain was expressed in Escherichia Coli.
Molecular weight Nativebovine bFGF is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated mass of approximatelykDa. Recombinantbovine basic FGFis a monomer protein, consisting of amino acids and migrates as an approximately kDa protein under reducing conditions.
Purity >98%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by the proliferation ofNR6R-3T3 fibroblasts, was found to be less than.1 ng/ml,corresponding tox106 IU/mg.
Protein Sequence MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGPKTG PGQKAILFLP MSAKS
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant bovine bFGFwas lyophilized from a.2 μm filtered PBS solution pH.5.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biological Process Angiogenesis
Biological Process Differentiation
Molecular function Developmental-protein
Molecular function Growth-factor
Molecular function Heparin-binding