| accession | P03969 |
| Source | Optimized DNA sequence encodingBovine Basic Fibroblast Growth Factor mature chain was expressed in Escherichia Coli. |
| Molecular weight | Nativebovine bFGF is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated mass of approximatelykDa. Recombinantbovine basic FGFis a disulfide-linked monomer protein, consisting of amino acids and migrates as an approximately kDa protein under reducing conditions. |
| Purity | >98%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED(50) was determined by the proliferation ofNR6R-3T3 fibroblasts, was found to be less than.1 ng/ml,corresponding tox106 IU/mg. |
| Protein Sequence | MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGPKTG PGQKAILFLP MSAKS |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant bovine bFGFwas lyophilized from a.2 μm filtered PBS solution pH.5. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Biological Process | Angiogenesis |
| Biological Process | Differentiation |
| Molecular function | Developmental-protein |
| Molecular function | Growth-factor |
| Molecular function | Heparin-binding |
bovine Basic Fibroblast Growth Factor Recombinant
$70.00 – $1,300.00
