Biotinylated SARS Spike RBD Protein Recombinant

//Biotinylated SARS Spike RBD Protein Recombinant

Biotinylated SARS Spike RBD Protein Recombinant

$542.00$1,805.00

SKU: KTAPHD9HB Category:

accession P59594


Source DNA sequence encoding SARS Spike RBD including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant SARS Spike RBD is a protein consisting of amino acid residues [Arg306-Phe527] ,The protein has a predicted MW of 27.9 kDa. Due to glycosylation, the protein migrates to 36-46 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant SARS Spike RBD was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor Q9BYF1 ACE2_HUMAN
Biological Process Virus-entry-into-host-cell
Biological Process Host-virus-interaction
Biological Process Virulence

Methods

Biotinylated SARS Spike RBD Protein Tris-Bis PAGE

  • Biotinylated SARS RBD on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated SARS Spike RBD Protein SEC-HPLC

  • The purity of Biotinylated SARS RBD is greater than 95% as determined by SEC-HPLC.