| accession | P59594 |
| Source | DNA sequence encoding SARS Spike RBD including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
| Molecular weight | Recombinant SARS Spike RBD is a protein consisting of amino acid residues [Arg306-Phe527] ,The protein has a predicted MW of 27.9 kDa. Due to glycosylation, the protein migrates to 36-46 kDa based on Tris-Bis PAGE result.. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | see methods section |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant SARS Spike RBD was lyophilized from 0.2 µm filtered PBS solution pH7.4 |
| Reconstitution | Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. |
| Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | Q9BYF1 ACE2_HUMAN |
| Biological Process | Virus-entry-into-host-cell |
| Biological Process | Host-virus-interaction |
| Biological Process | Virulence |
Methods
Biotinylated SARS Spike RBD Protein Tris-Bis PAGE
|
Biotinylated SARS Spike RBD Protein SEC-HPLC
|
