|Source||DNA sequence encoding SARS-COV-2 Spike S1 (Omicron B.1.1.529) including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
|Molecular weight||Recombinant SARS-COV-2 Spike S1 (Omicron B.1.1.529) is a protein consisting of amino acid residues [Gln14-Arg683(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H)] ,The protein has a predicted MW of 77.94 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result..|
|Purity||>95%, as determined by SDS-PAGE and HPLC|
|Biological Activity||see methods section|
|Endotoxin||Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).|
|Presentation||Recombinant SARS-COV-2 Spike S1 (Omicron B.1.1.529) was lyophilized from 0.2 µm filtered PBS solution pH7.4|
|Reconstitution||Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM|
|Storage||The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.|
|Usage||This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.|
Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) Protein ELISA Data