Biotinylated Rabbit Anti Human AITRL polyclonal, antigen affinity

///Biotinylated Rabbit Anti Human AITRL polyclonal, antigen affinity

Biotinylated Rabbit Anti Human AITRL polyclonal, antigen affinity

$190.00$230.00


accession Q9UNG2


Applications

ELISA:This antibody can be used at 0.2 – 1.0 μg/mL jointly with Human AITRL antibody to detect Human AITRL. The detection limit for recombinant Human AITRL is approximately 0.2 ng/well.

Source This antibody was produced in Rabbit immunized with recombinant Human AITRL.
Species reactivity Human
Purification The specific antibody was purified from Rabbit sera by using immobilized recombinant Human AITRL affinity chromatography.
Presentation Lyophilized from PBS, pH 7.2.
Storage The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C. Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for 12 months without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor O14763 TR10B_HUMAN
Interactor P01730 CD4_HUMAN
Interactor Q9Y5U5 TNR18_HUMAN
Biological Process Immunity
Molecular function Cytokine

Methods

Western Blot

  • The Recombinant immunogen was migrated by 12% SDS-PAGE and transferred to a 0.2 um PVDF membrane.
  • After blocking the membrane in blocking buffer (5% milk powder in 20 mM Tris-HCl pH 7.5, 500 mM NaCl, 0.1% (v/v) Tween 20 , the membrane was incubated with biotinylated primary antibody (0.1ug/ml) at 4°C overnight.
  • Enhanced chemiluminescence (ECL) reagents were used to visualize

Sandwich ELISA

  • 96-well maxisorp plates were coated with the capture antibody overnight, then blocked with 5% FCS in PBS for 1 hour, then washed.
  • Then, the protein standard was added, and plates were incubated for 2 hrs at 37°C.
  • Plates were washed and incubated with a biotinylated detection antibody for 1 hour at 37°C.
  • After washing, plates were incubated with HRP-conjugated streptavidin for 30 min at room temperature and washed again.
  • Plates were developed using the tetramethylbenzidine peroxidase substrate system and absorbance was measured at 450-615 nm using an automated plate reader.