Biotinylated Mouse PLAU/uPA Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
P06869
Source
DNA sequence encoding Mouse PLAU/uPA including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Mouse PLAU/uPA is a protein consisting of amino acid residues [Gly21-Phe433] ,The protein has a predicted MW of 49.00 kDa. Due to protein lysis and glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse PLAU/uPA was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Biotinylated Mouse PLAU/uPA Protein Tris-Bis PAGE
- Biotinylated Mouse PLAU on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Mouse PLAU/uPA Protein SEC-HPLC
- The purity of Biotinylated Mouse PLAU is greater than 95% as determined by SEC-HPLC.
Biotinylated Mouse PLAU/uPA Protein SPR Data
- Mouse uPAR isoform 1, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse PLAU, His-Avi Tag with an affinity constant of 0.19 μM as determined in SPR assay (Biacore T200).
