Biotinylated Mouse IL-2 Protein Recombinant

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Biotinylated Mouse IL-2 Protein Recombinant


SKU: KTRYAW4HB Categories: ,

accession P04351

Source DNA sequence encoding Mouse IL-2 including a N-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Mouse IL-2 is a protein consisting of amino acid residues [Ala21-Gln169] ,The protein has a predicted MW of 20.1 kDa. Due to glycosylation, the protein migrates to 27-30 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Mouse IL-2 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Biological Process Immunity
Molecular function Cytokine
Molecular function Growth-factor


Biotinylated Mouse IL-2 Protein Tris-Bis PAGE

  • Biotinylated Mouse IL-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Mouse IL-2 Protein SEC-HPLC

  • The purity of Biotinylated Mouse IL-2 is greater than 95% as determined by SEC-HPLC.

Biotinylated Mouse IL-2 Protein ELISA Data

  • Immobilized Mouse IL-2 R alpha, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 95.8ng/ml determined by ELISA.