Biotinylated Mouse GFRAL/GFR alpha-like Protein Recombinant
Category: Biotinylated proteins$618.00 – $2,062.00
Description
Accession
Q6SJE0
Source
DNA sequence encoding Mouse GFRAL/GFR alpha-like including a N-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Mouse GFRAL/GFR alpha-like is a protein consisting of amino acid residues [Gln20-Glu350] ,The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse GFRAL/GFR alpha-like was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Biotinylated Mouse GFRAL/GFR alpha-like Protein Tris-Bis PAGE
- Biotinylated Mouse GFRAL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Mouse GFRAL/GFR alpha-like Protein SEC-HPLC
- The purity of Biotinylated Mouse GFRAL is greater than 95% as determined by SEC-HPLC.
Biotinylated Mouse GFRAL/GFR alpha-like Protein ELISA Data
- Immobilized Biotinylated Mouse GFRAL, His Tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA.
