Biotinylated Mouse GARP&Latent TGF beta 1 Complex Protein Recombinant
Category: Biotinylated proteins$696.00 – $2,320.00
Description
Accession
G3XA59
Source
DNA sequence encoding Mouse GARP&Latent TGF beta 1 Complex including a N-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Mouse GARP&Latent TGF beta 1 Complex is a protein consisting of amino acid residues [Ala18-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)] ,The protein has a predicted MW of 66.6 kDa (GARP)&44.2 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 72-76 kDa (GARP)&13 kDa&45-47 kDa (Latent TGF beta 1) based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse GARP&Latent TGF beta 1 Complex was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Biotinylated Mouse GARP&Latent TGF beta 1 Complex Protein Tris-Bis PAGE
- Biotinylated Mouse GARP&Latent TGF beta 1 Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Mouse GARP&Latent TGF beta 1 Complex Protein SEC-HPLC
- The purity of Biotinylated Mouse GARP&Latent TGF beta 1 Complex is greater than 95% as determined by SEC-HPLC.
Biotinylated Mouse GARP&Latent TGF beta 1 Complex Protein ELISA Data
- Immobilized Mouse ITGAV&ITGB6, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse GARP&Latent TGF beta 1 Complex, His Tag with the EC50 of 0.12μg/ml determined by ELISA.
