Biotinylated Mouse CDCP1 Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
Q5U462
Source
DNA sequence encoding Mouse CDCP1 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Mouse CDCP1 is a protein consisting of amino acid residues [Arg25-Ala667] ,The protein has a predicted MW of 75.3 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse CDCP1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Biotinylated Mouse CDCP1 Protein Tris-Bis PAGE
- Biotinylated Mouse CDCP1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Mouse CDCP1 Protein SEC-HPLC
- The purity of Biotinylated Mouse CDCP1 is greater than 95% as determined by SEC-HPLC.
Biotinylated Mouse CDCP1 Protein SPR Data
- Anti-CDCP1 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Mouse CDCP-1, His-Avi Tag with an affinity constant of 0.74 μM as determined in SPR assay (Biacore T200).
