Biotinylated Mouse BCMA/TNFRSF17 Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
O88472
Source
DNA sequence encoding Mouse BCMA/TNFRSF17 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Mouse BCMA/TNFRSF17 is a protein consisting of amino acid residues [Met1-Thr49] ,The protein has a predicted MW of 8.5 kDa. Due to glycosylation, the protein migrates to 12-16 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse BCMA/TNFRSF17 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
Methods
Biotinylated Mouse BCMA/TNFRSF17 Protein Tris-Bis PAGE
- Biotinylated Mouse BCMA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Mouse BCMA/TNFRSF17 Protein SEC-HPLC
- The purity of Biotinylated Mouse BCMA is greater than 95% as determined by SEC-HPLC.
Biotinylated Mouse BCMA/TNFRSF17 Protein ELISA Data
- Immobilized Mouse APRIL, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 4.3ng/ml determined by ELISA.
Biotinylated Mouse BCMA/TNFRSF17 Protein SPR Data
- Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Mouse BCMA, His-Avi Tag with an affinity constant of 5.64 μM as determined in SPR assay (Biacore T200).
