Biotinylated Mouse BCMA/TNFRSF17 Protein Recombinant

//Biotinylated Mouse BCMA/TNFRSF17 Protein Recombinant

Biotinylated Mouse BCMA/TNFRSF17 Protein Recombinant

$542.00$1,805.00

SKU: KTTV7YUHB Category:

accession O88472


Source DNA sequence encoding Mouse BCMA/TNFRSF17 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Mouse BCMA/TNFRSF17 is a protein consisting of amino acid residues [Met1-Thr49] ,The protein has a predicted MW of 8.5 kDa. Due to glycosylation, the protein migrates to 12-16 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Mouse BCMA/TNFRSF17 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biological Process Immunity
Molecular function Receptor

Methods

Biotinylated Mouse BCMA/TNFRSF17 Protein Tris-Bis PAGE

  • Biotinylated Mouse BCMA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Mouse BCMA/TNFRSF17 Protein SEC-HPLC

  • The purity of Biotinylated Mouse BCMA is greater than 95% as determined by SEC-HPLC.

Biotinylated Mouse BCMA/TNFRSF17 Protein ELISA Data

  • Immobilized Mouse APRIL, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 4.3ng/ml determined by ELISA.

Biotinylated Mouse BCMA/TNFRSF17 Protein SPR Data

  • Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Mouse BCMA, His-Avi Tag with an affinity constant of 5.64 μM as determined in SPR assay (Biacore T200).