Biotinylated Mouse ALCAM/CD166 Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
Q61490
Source
DNA sequence encoding Mouse ALCAM/CD166 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Mouse ALCAM/CD166 is a protein consisting of amino acid residues [Tyr28-Lys527] ,The protein has a predicted MW of 58.9 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse ALCAM/CD166 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process
Methods
Biotinylated Mouse ALCAM/CD166 Protein Tris-Bis PAGE
- Biotinylated Mouse ALCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Mouse ALCAM/CD166 Protein SEC-HPLC
- The purity of Biotinylated Mouse ALCAM is greater than 95% as determined by SEC-HPLC.
