Biotinylated Human VEGF121 Protein Recombinant

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Biotinylated Human VEGF121 Protein Recombinant


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accession P15692

Source DNA sequence encoding Human VEGF121 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human VEGF121 is a protein consisting of amino acid residues [Ala27-Arg147] ,The protein has a predicted MW of 17 kDa. Due to glycosylation, the protein migrates to 18 kDa/22-25 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human VEGF121 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor O60462 NRP2_HUMAN
Interactor P07996 TSP1_HUMAN
Interactor O14786 NRP1_HUMAN
Interactor P01730 CD4_HUMAN
Interactor P17948 VGFR1_HUMAN
Interactor P35918 VGFR2_MOUSE
Interactor P35968 VGFR2_HUMAN
Interactor P49763 PLGF_HUMAN
Biological Process Angiogenesis
Biological Process Differentiation
Molecular function Developmental-protein
Molecular function Growth-factor
Molecular function Heparin-binding


Biotinylated Human VEGF121 Protein Tris-Bis PAGE

  • Biotinylated Human VEGF121 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human VEGF121 Protein ELISA Data

  • Immobilized Human VEGF R1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGF121, His Tag with the EC50 of 36.5ng/ml determined by ELISA.