| accession | P15692 |
| Source | DNA sequence encoding Human VEGF121 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
| Molecular weight | Recombinant Human VEGF121 is a protein consisting of amino acid residues [Ala27-Arg147] ,The protein has a predicted MW of 17 kDa. Due to glycosylation, the protein migrates to 18 kDa/22-25 kDa based on Tris-Bis PAGE result.. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | see methods section |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant Human VEGF121 was lyophilized from 0.2 µm filtered PBS solution pH7.4 |
| Reconstitution | Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. |
| Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | O60462 NRP2_HUMAN |
| Interactor | P07996 TSP1_HUMAN |
| Interactor | O14786 NRP1_HUMAN |
| Interactor | P01730 CD4_HUMAN |
| Interactor | P17948 VGFR1_HUMAN |
| Interactor | P35918 VGFR2_MOUSE |
| Interactor | P35968 VGFR2_HUMAN |
| Interactor | P49763 PLGF_HUMAN |
| Biological Process | Angiogenesis |
| Biological Process | Differentiation |
| Molecular function | Developmental-protein |
| Molecular function | Growth-factor |
| Molecular function | Heparin-binding |
Methods
Biotinylated Human VEGF121 Protein Tris-Bis PAGE
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Biotinylated Human VEGF121 Protein ELISA Data
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