Biotinylated Human VEGF R1/FLT-1 Protein Recombinant

//Biotinylated Human VEGF R1/FLT-1 Protein Recombinant

Biotinylated Human VEGF R1/FLT-1 Protein Recombinant

$542.00$1,805.00

SKU: KTLDSE4HB Category:

accession P17948


Source DNA sequence encoding Human VEGF R1/FLT-1 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human VEGF R1/FLT-1 is a protein consisting of amino acid residues [Ser27-Asn756] ,The protein has a predicted MW of 85.1 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human VEGF R1/FLT-1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P15692 VEGFA_HUMAN
Interactor P49763 PLGF_HUMAN
Biological Process Angiogenesis
Biological Process Chemotaxis
Biological Process Differentiation
Molecular function Developmental-protein
Molecular function Transferase
Molecular function Receptor

Methods

Biotinylated Human VEGF R1/FLT-1 Protein Tris-Bis PAGE

  • Biotinylated Human VEGF R1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human VEGF R1/FLT-1 Protein ELISA Data

  • Immobilized Human VEGF165 at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 26.9ng/ml determined by ELISA.

Biotinylated Human VEGF R1/FLT-1 Protein ELISA Data

  • Immobilized Human PGF, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 48ng/ml determined by ELISA.