Biotinylated Human TRAIL R4/TNFRSF10D Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
Q9UBN6
Source
DNA sequence encoding Human TRAIL R4/TNFRSF10D including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human TRAIL R4/TNFRSF10D is a protein consisting of amino acid residues [Ala56-His211] ,The protein has a predicted MW of 19.4 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human TRAIL R4/TNFRSF10D was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Molecular function
Methods
Biotinylated Human TRAIL R4/TNFRSF10D Protein Tris-Bis PAGE
- Biotinylated Human TRAIL R4 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Biotinylated Human TRAIL R4/TNFRSF10D Protein SEC-HPLC
- The purity of Biotinylated Human TRAIL R4 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human TRAIL R4/TNFRSF10D Protein ELISA Data
- Immobilized Human TRAIL, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TRAIL R4, His Tag with the EC50 of 0.14μg/ml determined by ELISA.
Biotinylated Human TRAIL R4/TNFRSF10D Protein SPR Data
- Biotinylated Human TRAIL R4, His-Avi Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.135 nM as determined in SPR assay (Biacore T200).
