Biotinylated Human TGF Beta-1 Recombinant
$450.00 – $3,900.00
| accession | P01137 |
| Source | DNA sequence encoding Human Transforming Growth Factor Beta-1 mature chain was expressed in HEK293 cells fused to C terminal AVI tag |
| Molecular weight | Recombinant TGF-beta 1 is a disulfide-linked homodimer protein consisting of 2x113 amino acid residue subunits. TGF-beta 1 migrates as an approximately 12 - 13 kDa protein under reducing conditions and as 26kDa under nonreducing conditions |
| Biotinylateion | TGFB1 is biotinylated in-vitro at a single site using the AVItagTM biotinylation technology. . |
| Purity | >90%, as determined by SDS-PAGE and HPLC. |
| Biological activity | The ED(50) was determined by in a functional ELISA assay binding human TGFBR2 (cat#RKP37173) at 1ug/ml and was determined to be 15ng/ml. |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Biotinylated Recombinant TGF-b1 is lyophilized from 0.2 μm filtered PBS solution pH 7.4. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P01730 CD4_HUMAN |
| Interactor | P37023 ACVL1_HUMAN |
| Interactor | P18564 |
| Interactor | P36897 TGFR1_HUMAN |
| Interactor | P37173 TGFR2_HUMAN |
| Interactor | Q03167 |
| Interactor | Q14392 LRC32_HUMAN |
| Interactor | Q14766 |
| Interactor | Q62356 FSTL1_MOUSE |
| Molecular function | Growth-factor |
| Molecular function | Mitogen |
Methods
Biotinylated TGFB-1 protein PAGE
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Biotinylated TGFB-1 ELISA
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