Biotinylated Human TGF Beta-1 Recombinant
Categories: Biotinylated proteinsCytokinesRecombinant Human Cytokines$450.00 – $3,900.00
Description
Accession
P01137
Source
DNA sequence encoding Human Transforming Growth Factor Beta-1 mature chain was expressed in HEK293 cells fused to C terminal AVI tag
Molecular weight
Recombinant TGF-beta 1 is a disulfide-linked homodimer protein consisting of 2x113 amino acid residue subunits. TGF-beta 1 migrates as an approximately 12 - 13 kDa protein under reducing conditions and as 26kDa under nonreducing conditions
Biotinylateion
TGFB1 is biotinylated in-vitro at a single site using the AVItagTM biotinylation technology. .
Purity
>90%, as determined by SDS-PAGE and HPLC.
Biological activity
The ED(50) was determined by in a functional ELISA assay binding human TGFBR2 (cat#RKP37173) at 1ug/ml and was determined to be 15ng/ml.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Biotinylated Recombinant TGF-b1 is lyophilized from 0.2 μm filtered PBS solution pH 7.4.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
P18564
Interactor
Interactor
Interactor
Q03167
Interactor
Interactor
Q14766
Interactor
Molecular function
Molecular function
Methods
Biotinylated TGFB-1 protein PAGE
- 1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.
Biotinylated TGFB-1 ELISA
- Immobilized Biotinylated Human TGF Beta1 at 1ug/ml (100ul/Well). Response curve for TGFR2 (cat#RKP37173) with alinear range of 2-100 ng/ml was determined by ELISA
