Biotinylated Human TGF Beta-1 Recombinant

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Biotinylated Human TGF Beta-1 Recombinant

$450.00$3,900.00


accession P01137


Source DNA sequence encoding Human Transforming Growth Factor Beta-1 mature chain was expressed in HEK293 cells fused to C terminal AVI tag
Molecular weight Recombinant TGF-beta 1 is a disulfide-linked homodimer protein consisting of 2x113 amino acid residue subunits. TGF-beta 1 migrates as an approximately 12 - 13 kDa protein under reducing conditions and as 26kDa under nonreducing conditions
Biotinylateion TGFB1 is biotinylated in-vitro at a single site using the AVItagTM biotinylation technology. .
Purity >90%, as determined by SDS-PAGE and HPLC.
Biological activity The ED(50) was determined by in a functional ELISA assay binding human TGFBR2 (cat#RKP37173) at 1ug/ml and was determined to be 15ng/ml.
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Biotinylated Recombinant TGF-b1 is lyophilized from 0.2 μm filtered PBS solution pH 7.4.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P01730 CD4_HUMAN
Interactor P37023 ACVL1_HUMAN
Interactor P18564
Interactor P36897 TGFR1_HUMAN
Interactor P37173 TGFR2_HUMAN
Interactor Q03167
Interactor Q14392 LRC32_HUMAN
Interactor Q14766
Interactor Q62356 FSTL1_MOUSE
Molecular function Growth-factor
Molecular function Mitogen

Methods

Biotinylated TGFB-1 protein PAGE

  • 1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.

Biotinylated TGFB-1 ELISA

  • Immobilized Biotinylated Human TGF Beta1 at 1ug/ml (100ul/Well). Response curve for TGFR2 (cat#RKP37173) with alinear range of 2-100 ng/ml was determined by ELISA