Biotinylated Human Siglec-8 Protein Recombinant

//Biotinylated Human Siglec-8 Protein Recombinant

Biotinylated Human Siglec-8 Protein Recombinant


SKU: KTBZWX0HB Category:

accession Q9NYZ4

Source DNA sequence encoding Human Siglec-8 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human Siglec-8 is a protein consisting of amino acid residues [Met17-Ala363] ,The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human Siglec-8 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biotinylated Human Siglec-8 Protein Tris-Bis PAGE

  • Biotinylated Human Siglec-8 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.