Biotinylated Human Siglec-10 (R119A) Protein Recombinant

//Biotinylated Human Siglec-10 (R119A) Protein Recombinant

Biotinylated Human Siglec-10 (R119A) Protein Recombinant



accession Q96LC7

Source DNA sequence encoding Human Siglec-10 (R119A) including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human Siglec-10 (R119A) is a protein consisting of amino acid residues [Met17-Thr546] ,The protein has a predicted MW of 62.64 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human Siglec-10 (R119A) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biotinylated Human Siglec-10 (R119A) Protein Tris-Bis PAGE

  • Biotinylated Human Siglec-10 (R119A) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human Siglec-10 (R119A) Protein SEC-HPLC

  • The purity of Biotinylated Human Siglec-10 (R119A) is greater than 95% as determined by SEC-HPLC.

Biotinylated Human Siglec-10 (R119A) Protein ELISA Data

  • Immobilized Anti-Siglec-10 Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-10 (R119A) , His Tag with the EC50 of 61.7ng/ml determined by ELISA.