Biotinylated Human PD-L1/B7-H1 Protein Recombinant

//Biotinylated Human PD-L1/B7-H1 Protein Recombinant

Biotinylated Human PD-L1/B7-H1 Protein Recombinant


SKU: KTG2DE0HB Category:

accession Q9NZQ7

Source DNA sequence encoding Human PD-L1/B7-H1 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human PD-L1/B7-H1 is a protein consisting of amino acid residues [Phe19-Arg238] ,The protein has a predicted MW of 28.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human PD-L1/B7-H1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor Q02242 PDCD1_MOUSE
Molecular function Receptor


Biotinylated Human PD-L1/B7-H1 Protein Tris-Bis PAGE

  • Biotinylated Human PD-L1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human PD-L1/B7-H1 Protein SEC-HPLC

  • The purity of Biotinylated Human PD-L1 is greater than 95% as determined by SEC-HPLC.

Biotinylated Human PD-L1/B7-H1 Protein ELISA Data

  • Immobilized Anti-PD-L1 Antibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human PD-L1, His Tag with the EC50 of 2.8ng/ml determined by ELISA.